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Want to buy a NovaSeq 6000 for sequencing?

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  • Want to buy a NovaSeq 6000 for sequencing?

    Saw within the FTDNA website advertising an image of a NovaSeq 6000, used in sequencing and was wondering what exactly it was. Some people might be interested in this stuff, the technical side of machines which do the sequencing and I guess some do not. If interested - read on.

    I got all of the info below from the following x2 URLs if you wish to dig deeper.

    First link says the new machines (well they were new back then in 2017) are also "easier to use, with the number of steps in its workflow cut from 38 to 8.
    The machine has been made more idiot-proof, with RFID chips checking to make sure components are loaded properly."
    - Good to know it is more idiot proof.

    "The NovaSeq 5000 and 6000 cost $850,000 and $985,000, respectively." - That's one reason prices for some tests are not cheap.

    "The new DNA sequencers, called the NovaSeq 5000 and the NovaSeq 6000, could be about 70% faster than existing machines"

    DNA sequencers actually only read small bits of DNA, which are assembled like a puzzle. The Illumina machine is still stuck at 150 base pairs (pairs of DNA letters).
    But some new technologies are allowing much longer read lengths– at a much higher cost per DNA base pair.

    The machine itself weighs alot.
    I put in both imperial and metric measurements so all can relate.

    Crated Weight : 628kg (1385lb)
    Weight : 481kg (1059lb)

    It has an integrated motherboard/chip setup, these are mainly used for industrial purposes.

    Instrument control computer Base Unit:
    PortwellWADE-8022 motherboard with Intel i74700EQ CPU 6M Cache, up to 3.40 GHz
    Memory: 2×8GB DDR3L SODIMM
    Solid-State Drive:256GB mSATA
    Operating System: Windows
    -Hopefully none of the delays to tests were caused by Windows updates..... :-)

    Width 80.0cm(31.5in)
    Depth 94.5cm(37.2in)
    Height 165.6cm(65.2in)with monitor

    It does seem to have a specific operating environment.

    Temperature:19°C (66.2°F) to 25°C (77°F) (ie 22°C ±3°C) <2°C change per hour
    Humidity - Non condensing 20–80% relative humidity
    Altitude:Below 2000 meters(6500 feet)
    Ventilation:Maximum of 8530BTU / and average 6000BTU/h
    (BTU = British Thermal Unit)
    200–240 VAC 50/60Hz,16A,singlephase,2500W
    -It does seems to use a bit of power - drawing 16 Amps and 2.5KW of power. It does come with an Uninterruptible Power Supply which also must be fairly big/heavy.

    Not sure what the lasers are used for, but there are four of them - don't look at them or you might "kentucky fry" your eyeballs.
    Laser 532nm,660nm,780nm,790nm

    Dedicated - 1Gb connection between the instrument and data management system.
    There must be heaps of data obtained from this thing, and also need equally large database system and management system to store all of that data over a fast network link.
    Hopefully they have a comprehensive backup/restore regime if something goes wrong in FTDNA land.

    The samples themselves must be stored in a controlled (possibly refrigerated) environment. It must take up alot of room all of those samples through the years.

    "Nova Seq 6000 System generates from 80Gb and 800M reads to 3Tb and 10B reads of data in single flow cell mode. In dual flow cell mode,output can be up to 6Tb and 20B reads."

    Blimey - That means they would go through alot of hard drives, as SSDs are not quite large enough or cost effective for storage. Biggest HDD currently on the market is approx 16TB (eg Seagate Exos ST16000NM001G - huge)

    It is interesting the brochure it says "For Research Use Only.Not for use in diagnostic procedures"
    I guess they have a more expensive model to do that?

    So if you want to buy one of these for your backyard sequencing hobby, might need to attach lightning rods to your roof to get extra power through storms, and you will need a temperature controlled environment and be in a location that is not over 2000 meters(6500 feet) above sea level - oh and up to a million in spare change !