Announcement

Collapse
No announcement yet.

Batch 174 Created

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Originally posted by VanLaar
    It says here (dna-fingerprint information) that for DYS385 in panel 2 different primers are used. I don't know much about the technical/lab side of dna testing so I don't know what that means.
    There should be no difference in the reported result, but I think that the primers in Panel 2 work well on weaker DNA samples (for which the original primers from Panel 1 sometimes fail).

    http://www.ncbi.nlm.nih.gov/entrez/q...&dopt=Abstract

    Comment


    • What are the odds that all remaining batch 174 results will actually come in today? I've been waiting so long.......

      John

      Comment


      • In other batches, I have gotten my results early or on time. However, since this batch has been jinxed from the start (22 Nov 2006), I have learned not to "hold my breath".....

        Comment


        • Another due date come and gone....Will results ever arrive or will this batch 174 just fade away with time?

          Comment


          • I just got my DYS 413 results. Now I'm just waiting for DYS 464X and my batch 191 results which are also late.

            John

            Comment


            • I finally got my DYS413 results today and it feels good! About time!

              Comment


              • Still waiting for my DYS462.....Maybe in a couple of more months.....

                Comment


                • Still waiting for the elusive DYS462 marker from this batch....Hope results are in when I get back from vacation in a few weeks. Hope they resolve the screwup on the re-run of DYS 385 also...

                  Comment


                  • Originally posted by marvallen
                    Still waiting for the elusive DYS462 marker from this batch....Hope results are in when I get back from vacation in a few weeks. Hope they resolve the screwup on the re-run of DYS 385 also...
                    DYS435(DYS435) 04.06.2007 174
                    DYS726(DYS726) 04.06.2007 174
                    Y-GGAAT-1B07(Y-GGAAT-1B07) 04.06.2007 175

                    Comment


                    • Five months late today and still waiting for some results No new due date either....

                      John

                      Comment


                      • Finally got all of my results reported. DYS42 = 13. All re-checks done, all problems resolved. Think I'm done for a few weeks. Just wish FTDNA would add the new markers to their surname pages.....

                        Comment


                        • Congratulations Marv,

                          I am still waiting for DYS464X. Will the last batch 174 results ever come in....

                          John

                          Comment


                          • John,

                            You must be the last one left holding the bag, awaiting results from this Batch. Good luck!!! Since I sweated out the wait on this one down to "next to last", I'm going to "give it a rest" for a while before venturing back into the "DNA testing waters"....

                            Comment


                            • I have finally received my last batch 174 results.

                              However, the result does not match my prior result completely. Before my DYS464 result was 14, 15, 15, 15. My new result is 14, 14.3, 15, 15. I did not know that you could get a partial on DYS464.

                              John

                              Comment


                              • Apparently 13% of samples in an Austrian study showed "intermediate alleles" like 14.3. Who knew?

                                "Titre du document / Document title
                                Molecular characterization and Austrian Caucasian population data of the multi-copy Y-chromosomal STR DYS464
                                Auteur(s) / Author(s)
                                BERGER Burkhard (1) ; NIEDERSTÄTTER Harald (1) ; BRANDSTÄTTER Anita (1) ; PARSON Walther (1) ;
                                Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
                                (1) Institute of Legal Medicine, University of Innsbruck, Müllerstrasse 44, 6020 Innsbruck, AUTRICHE

                                Résumé / Abstract
                                DYS464 is a multi-copy STR system with four positions on the Y-chromosome (DYS464a, b, c, and d) which was recently identified and characterized [Forensic Sci. Int. 130 (2002) 97]. The aims of our study were to perform a population study, to estimate the mutation rate and an extensive sequence analysis in order to confirm the nomenclature. Fourteen different alleles were found in an Austrian population sample with an allele length varying from 9 to 19 repeats. All alleles were cloned and sequenced. Alleles 9-19 showed the general repeat structure (CCTT)[n]... (CCTT)[2]... (CCTT)[3]... (CCTT)[4]... (CCTT)[2]... (CCTT)[2]. The nomenclature is based on the number of repeated units of the variable (CCTT)[n]-stretch only. In 13% of the samples intermediate alleles, namely 14.3A, 14.3B and 15.3 were detected. In these alleles the variable repeat block is interrupted by a CTT motif (14.3A: (CCTT)[3]CTT(CCTT)[11]; 14.3B and 15.3: (CCTT)[7]CTT(CCTT)[7/8]). A comparison with GenBank entries revealed the existence of a length variant due to a deletion of one cytosine in the 5' flanking region of the first repeat block. We designed an alternative forward primer to circumvent possible ambiguities in the allele designation. A total of 54 different genotypes were identified in 135 men corresponding to a discrimination capacity (DC) of 40% and a gene diversity (GD) of 0.97. These values are much higher than those of other Y-chromosomal short tandem repeats (Y-SRs). DYS464 has the same haplotype diversity (HD) as the combination of the five Y-STR loci with the lowest gene diversities of the Y-STR core set. On the other hand, a combination of the three most diverse loci (DYS464, DYS385 and DYS390) has the same capacity to distinguish between paternal lineages than the complete minimal haplotype (minHT) consisting of eight Y-STR loci. In our population sample the addition of DYS464 to the minHT increases the number of different haplotypes from 110 to 122. The mutation-rate estimate based on the 70 meioses analyzed amounts to 2.86 x 10[-2] (95% confidence interval 3.5 x 10[-3] to 9.95 x 10[-2]). This value is approximately 10 times higher than the average mutation-rate estimate for Y-STRs."


                                John

                                Comment

                                Working...
                                X